Laboratory Exercises in Microbiology by Robert A. Pollack (English) Paperback Bo

Description: Laboratory Exercises in Microbiology by Robert A. Pollack, Lorraine Findlay, Walter Mondschein, R. Ronald Modesto Preceded by Laboratory exercises in microbiology / Robert A. Pollack ... [et al.]. 4th ed. 2011. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description The Laboratory Exercises in Microbiology, 5e by Pollack, et al. presents exercises and experiments covered in a 1 or 2-semester undergraduate microbiology laboratory course for allied health students. The labs are introduced in a clear and concise manner, while maintaining a student-friendly tone. The manual contains a variety of interactive activities and experiments that teach students the basic concepts of microbiology. The 5th edition contains new and updated labs that cover a wide array of topics, including identification of microbes, microbial biochemistry, medical microbiology, food microbiology, and environmental microbiology. Table of Contents Preface iii Introduction: Laboratory Operations and Safety xiii Basic Rules for Microbiology Laboratory Safety xv Part 1 General Microscopy and Aseptic Technique 1 Laboratory Safety: Introduction to the Microscope 3 Laboratory Safety and Procedures 4 Laboratory Procedures 4 Simple Stain Technique 5 The Microscope 6 Microscope Components (Plate 1) 7 Eukaryotic Versus Prokaryotic Cells (Plates 2A, 2B) 10 Laboratory Cleanup 10 Microscope Cleanup 10 Discards 11 General Cleanup 11 Speaking of Safety 11 2 Transfer and Isolation Techniques, Microbes in the Environment 17 Transfer Technique 17 Tube-to-Tube Transfers 18 Basic Aseptic Technique Procedures 18 Isolation Techniques: Streak Plate and Pour Plate (Optional) 22 Streak Plate (Plate 3) 22 Prepare the Following Streak Plates Using the Cultures Provided 25 To Lap or Not to Lap— That is the Question 25 Pour Plate (Plate 4) Optional, May be Done as a Demonstration 25 Microbes in the Environment 26 Human Environment: Procedure 27 Classroom/School Environment: Procedure 27 Incubation 27 Results 27 Laboratory Cleanup 28 Discards 28 General Cleanup 28 Part 2 Microbial Morphology, Differential Stains 3 Cultural and Cellular Morphology 33 Cultural Characteristics of Bacteria 33 Procedure: Plates (Plate 5) 34 Procedure: Agar Slants 35 Procedure: Broth (Plate 6) 35 Microbial Cellular Morphology 35 Smear Preparation 36 Broth Preparation 37 Agar Slant and Plate Preparation 37 Simple Stain Procedure 37 Results from the Simple Stain Procedure (Plates 7–16) 38 Results from Prepared Slide 38 Laboratory Cleanup 38 4 Bacterial Growth 43 Factors Needed for Bacterial Growth 44 Measuring Bacterial Growth 45 Procedure for Preparation of Spread Plate 45 Procedure for Demonstration of a Bacterial Growth Curve 47 5 Gram Stain and Acid-Fast Stain 53 The Gram Stain 53 The Gram Stain Technique (Traditional Method) (Plate 17A, B, C) 54 Gram Stain: (Alternative Method) 55 The Acid-Fast Stain Technique (Plate 18) 56 Acid-Fast Technique 56 Laboratory Cleanup 58 Incubation 58 Discards 58 General Cleanup 58 Slides and Microscopes 58 6 Endospore Stain, Capsule Stain, and the Hanging Drop Technique 61 The Endospore Stain 61 The Spore Stain Technique (Plates 19, 20) 62 Results 63 The Capsule Stain 63 The Capsule Stain Technique (Plate 21) 63 The Hanging Drop Technique 64 Results 64 7 Fungi 69 The Fungi 69 Results 72 8 Viruses—Visualization and Enumeration 77 Viruses 77 Bacteriophage Enumeration 78 Results 80 9 Parasitology 85 Part 3 Microbial Control and Biochemistry 10 Microbial Sensitivity Testing 97 Part 1: Physical Methods Ultraviolet Light Sensitivity 98 Sterilization by the Use of UV Light 98 The Penetrating Power of UV Light (Plate 34) 99 Heat Sensitivity (may be done as an alternative to the UV light procedure) 101 Effect of Cold Temperature and Slow Freezing (Optional) 102 Part 2: Chemical Methods Chemical Sensitivity 102 Chemotherapeutic Agent Testing: The Kirby–Bauer Plate 103 The Kirby–Bauer Technique 104 Results 107 11 Bacterial Biochemistry 113 Carbohydrate Metabolizing Enzymes 114 Results 115 Carbohydrate Fermentation (Phenol Red Broth) Tubes (Plate 37) 115 Amino Acid and Nitrogen Metabolism 116 Results 117 Decarboxylase Tubes (Plate 42) 117 12 Gas Requirements of Microorganisms 123 Growth of Anaerobes (may be done as a demonstration) 124 Demonstration of Catalase 125 13 Specialized Media 129 Examples 130 Inoculation of Blood Agar Plates for Demonstration of Alpha Hemolysis and Transport Medium 131 Inoculation of Blood, Phenylethyl Alcohol, Mannitol Salt, Macconkey, and Eosin–Methylene Blue Agar Plates 132 Results 133 Inoculation of Triple Sugar Iron Agar and Sulfide–Indole Motility Medium 133 Results 134 Results of Transport Medium 134 Part 4 Medical Microbiology 14 Genetics 143 Ames Test 144 UV Light Procedure 145 Kirby–Bauer Procedure 145 Results: Ames Test (Plate 55) 145 Results: UV Light Procedure (Plate 35) 146 Results: Kirby–Bauer Test (Plates 36, 55) 146 15 Epidemiology 149 Handwashing Procedure (Done with Three Students) 150 Results: Handwashing (Plate 56) 151 Fomite and Direct Transmission of Microbes 151 Results: Fomite and Direct Transmission 152 Airborne Infections: Cough and Sneeze Plates 152 Results: Airborne and Cough/Sneeze Plates 153 Microbes in Makeup (Optional) 153 Results: Microbes in Makeup 153 16 Specimen-Handling Protocols 159 Quantitative Urinalysis 160 Results: Urine Samples 162 Specimens from the Gastrointestinal Tract 163 Results: GI Tract Isolation Technique 163 17 Specific Laboratory Tests 169 Catalase Test 171 Alternative Method (to be done at the end of this exercise) 171 Results: Catalase Test (Plate 46) 171 Bacitracin Sensitivity—Demonstration 171 Results: Bacitracin Sensitivity (Plate 57) 172 Oxidase Test 172 Results: Oxidase Test (Plate 59) 172 Coagulase Test 172 Rapid Slide Test 172 Tube Test 172 Results: Coagulase Tube Test (Plate 60) 172 Novobiocin Sensitivity 172 Results: Novobiocin Sensitivity 173 Camp Test (Plate 58) 173 Results: Camp 173 18 Serology 179 Elisa Test: Test for Toxins A and B from Clostridium Difficile 180 Results 182 Rapid Identification of Group A Antigen 182 Results 184 Differentiation of Streptococci Using Latex Agglutination 184 Slidex Strepto-Kit® 184 Results 186 Procedure Controls 186 Part 5 Identification of a Bacterial Unknown 19 Identification of Enteric Pathogens: Traditional Methods 191 Results 194 IMVIC Test (See Plate 61) 194 Results of Gelatin Hydrolysis 194 Results of the Oxidase Test (See Plate 59) 194 20 Identification of Enteric Pathogens: Rapid Identification Methods 201 EnteroPluri Test (Plate 62) 201 Compartment 1: Glucose Fermentation and Gas Production 202 Compartments 2 and 3: Lysine and Ornithine Decarboxylation 202 Compartment 4: Sulfide and Indole Production 202 Compartments 5–8: Adonitol, Lactose, Arabinose, and Sorbitol Fermentation 202 Compartment 9: Voges–Proskauer 202 Compartment 10: Phenylalanine Deaminase and Dulcitol 202 Compartment 11: Urea 202 Compartment 12: Citrate 202 Api® 20 E System (Plate 63) 203 Microtube 1: Ortho-Nitrophenyl-Beta-D-Ortho-Nitrophenyl-Beta-D-Galactopyranoside (Onpg) 203 Microtubes 2–4: Arginine Dihydrolase (Decarboxylase), Lysine Decarboxylase, and Ornithine Decarboxylase (Adh, Ldc, Odc) 203 Microtube 5: Citrate (Cit) 203 Microtube 6: Sulfide (H2s) 203 Microtube 7: Urea (Ure) 203 Microtube 8: Tryptophan Deaminase (Tda) 203 Microtube 9: Indole (Ind) 203 Microtube 10: Voges–Proskauer (Vp) 203 Microtube 11: Gelatin Hydrolysis (Gel) 203 Microtubes 12–20: Utilization of the Carbohydrates Glucose, Mannitol, Inositol, Sorbitol, Rhamnose, Saccharose (Sucrose), Melibiose, Amygdalin, and Arabinose (Glu, Man, Ino, Sor, Rha, Sac, Mel, Amy, And Ara) 203 Inoculation of Rapid Identification Media 204 Inoculation of EnteroPluri Test 204 Inoculation of the Api 20 E System 204 Results 205 21 Identification of a Bacterial Unknown: The Gram-Negative Unknown 209 Review 209 Comparison Chart 210 Flowchart 210 Computer Identification 210 ID Value Worksheet for EnteroPluri Test II 212 Results 213 22 Identification of a Bacterial Unknown: The Gram-Positive Cocci 217 Part 6 Food and Environmental Microbiology 23 Identification and Quantitation of Microbial Numbers in a Water Sample 225 Presumptive Test: Analysis of a Contaminated Water Sample for the Presence of Coliforms 226 Results 227 Quantitation of Microbial Number in a Water Sample 227 Results (Plate 64) 228 24 Identification of Microbes in Beef and Poultry and the Quantitation of Microbial Numbers 231 Microbial Presence in a Food Product and Colony Counts 232 Results 233 Recognition of Organism Genera 233 25 Soil Microbiology 237 Part 1: The Recovery of Microorganisms from Soil 238 Results 239 Isolation Of Soil Bacteria 239 Results 239 Part 2: Isolation of Soil Bacteriophages of Bacillus Subtilis 239 Results 240 26 Microbial Ecology 245 Competition Between Bacteria 246 Results 246 Bacteriocin Production 246 Results 247 Bacterial–Fungal Interaction (Plate 65) 247 Results 247 27 Biofilms 251 Growth of Dental Plaque in situ 252 Results 253 Soil Biofilm 253 Results 253 Growth of Bacterial Biofilms in vitro 253 Results 254 Bibliography 261 Answer Key for the Exercise Pre-Tests 263 Appendix 1 Flow Charts and Comparison Charts for the Identification of Enteric Gram Negative Rods Using Traditional Testing Procedures 265 Appendix 2 Flow Chart for the Identification of Enterobacteriaceae based on the API 20 E Rapid Identification System 269 Appendix 3 Gram-Positive Flow Chart 275 Photo Credit List 277 Index 279 Photographic Atlas PA-1 Details ISBN1119462665 Publisher John Wiley & Sons Inc Edition 5th ISBN-10 1119462665 ISBN-13 9781119462668 Format Paperback Imprint John Wiley & Sons Inc Place of Publication New York Country of Publication United States DEWEY 579.078 Pages 320 Language English AU Release Date 2018-07-11 NZ Release Date 2018-07-11 UK Release Date 2018-07-11 Author R. Ronald Modesto Edition Description 5th edition Year 2021 Publication Date 2021-03-02 Replaces 9781118135259 Audience Tertiary & Higher Education US Release Date 2021-03-02 We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:137355357;

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